Orientation Dependence in Homologous Recombination
نویسندگان
چکیده
منابع مشابه
Orientation dependence in homologous recombination.
Homologous recombination was investigated in Escherichia coli with two plasmids, each carrying the homologous region (two defective neo genes, one with an amino-end deletion and the other with a carboxyl-end deletion) in either direct or inverted orientation. Recombination efficiency was measured in recBC sbcBC and recBC sbcA strains in three ways. First, we measured the frequency of cells carr...
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The frequency of homologous recombination is believed to be a linear function of the length (N bp) of homology between DNAs. Here, the N intercept is believed to be determined by a threshold length below which some physical constraint is effective. In the mammalian gene targeting systems, however, the frequency depends more steeply than linearly on the homology length. To explain both the linea...
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ژورنال
عنوان ژورنال: Genetics
سال: 1996
ISSN: 1943-2631
DOI: 10.1093/genetics/143.1.27